Home Tools

G-418 disulfate

Catalog No. T6512 CAS 108321-42-2

Synonyms: Geneticin, G418 Sulfate, Antibiotic G-418 sulfate, Geneticin sulfate

G-418 disulfate (Geneticin sulfate) is an aminoglycoside antibiotic, an inhibitor of protein synthesis with eukaryotic selectivity. G-418 disulfate blocks peptide synthesis by inhibiting peptide chain elongation.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

G-418 disulfate, CAS 108321-42-2

Pack Size	Availability	Price/USD
500 mg	In stock	$ 42.00
1 g	In stock	$ 50.00
5 g	In stock	$ 158.00

Bulk Inquiry

Select Batch

Purity: 99.9%

Purity: 99.88%

Purity: 99.85%

Purity: 99.78%

Purity: 99.72%

Purity: 99.71%

Purity: 99.68%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	G-418 disulfate (Geneticin sulfate) is an aminoglycoside antibiotic, an inhibitor of protein synthesis with eukaryotic selectivity. G-418 disulfate blocks peptide synthesis by inhibiting peptide chain elongation.
In vitro	Continuous administration of G418 at doses ranging from 40 to 80 mg/kg for three consecutive days is sufficient to eradicate all untransfected Brucella bacterium parasites in infected mice.
In vivo	At concentrations ranging from 1 to 300 micrograms/ml, G418 inhibits a variety of prokaryotic and eukaryotic organisms. Typically, concentrations of 5 mg/L or lower are effective against bacteria and algae, while a concentration of 400 mg/L is used for the selection of mammalian cells, and a maintenance concentration for mammalian cells is set at 200 mg/L.

Synonyms	Geneticin, G418 Sulfate, Antibiotic G-418 sulfate, Geneticin sulfate
Molecular Weight	692.71
Formula	C20H40N4O10·2H2O4S
CAS No.	108321-42-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 43.31mM

DMSO: Insoluble

References and Literature

1. Delrue I, et al. Determination of the Selection Capacity of Antibiotics for Gene Selection. Biotechnol J. 2018 Aug;13(8):e1700747. 2. Ye G, et al. The FAP α -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway. Acta Pharm Sin B. 2022 Mar;12(3):1288-1304. 3. Nasonovs A, et al. A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids. PLoS One. 2021 Mar 25;16(3):e0249047. 4. Heier CR, et al. Translational readthrough by the aminoglycoside geneticin (G418) modulates SMN stability in vitro and improves motor function in SMA mice in vivo. Hum Mol Genet. 2009 Apr 1;18(7):1310-22. 5. Murphy NB, et al. Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants. Antimicrob Agents Chemother. 1993 May;37(5):1167-70.

Citations

1. Shao C S, Zhou X H, Miao Y H, et al. In-situ observation of mitochondrial biogenesis as the early event of apoptosis. Iscience. 2021, 24(9): 103038. 2. Yan R, Xie E, Li Y, et al. The structure of erastin-bound xCT–4F2hc complex reveals molecular mechanisms underlying erastin-induced ferroptosis. Cell Research. 2022-32（7）P1-4 3. Ye G, Huang M, Li Y, et al. The FAPα-activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway. Acta Pharmaceutica Sinica B. 2021 4. Shao C S, Zhou X H, Miao Y H, et al. In situ observation of mitochondrial biogenesis as the early event of apoptosis. Iscience. 2021, 24(9): 103038 5. Wang W, Zhang M, Huang Z, et al. Knockdown of CXCL5 inhibits the invasion, metastasis and stemness of bladder cancer lung metastatic cells by downregulating CD44. Anti-Cancer Drugs. 2022, 33(1): e103-e112. 6. Wang W, Zhang M, Huang Z, et al. Knockdown of CXCL5 inhibits the invasion, metastasis and stemness of bladder cancer lung metastatic cells by downregulating CD44. Anti-Cancer Drugs. 2022, 33(1): e103-e112. 7. Cheng G, An F, Cao Z, et al.DPY30 promotes the growth and survival of osteosarcoma cell by regulating the PI3K/AKT signal pathway.European Journal of Histochemistry.2023, 67(1).

Related compound libraries

This product is contained In the following compound libraries：

Microbial Natural Product Library Bioactive Compounds Library Max FDA-Approved & Pharmacopeia Drug Library Natural Product Library for HTS Anti-Infection Compound Library Immunology/Inflammation Compound Library Anti-infective Natural Product Library Bioactive Compound Library Natural Product Library Anti-Bacterial Compound Library

Related Products

Related compounds with same targets

(-)-Asarinin Isoimperatorin α-Vitamin E Etzadroxil Ceftezole sodium Netilmicin sulfate Azidamfenicol 2-Heptanol

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

G-418 disulfate 108321-42-2 Microbiology/Virology Antibacterial Antibiotic synthesis eukaryotic Antibiotic G-418 Geneticin Sulfate G418 Antibiotic G 418 Antibiotic G418 Sulfate Antibiotic G418 Geneticin Bacterial Antibiotic G 418 Sulfate Inhibitor G 418 Sulfate protein G 418 disulfate inhibit G418 Sulfate G-418 Antibiotic G-418 sulfate G-418 Disulfate cells G 418 Antibiotic G-418 Sulfate G418 disulfate Geneticin sulfate G418 Disulfate G 418 Disulfate G-418 Sulfate inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.